In-Vitro release and pharmacological study of synthesized valproic acid-dextran conjugate

Abstract

The valproic acid - dextran conjugate was synthesized by preparing valproic acid acyl imidazole with N, N carbonyldiimidazole and in- situ condensation with dextran of molecular weight 110,000. The degree of substitution was estimated to be 16% and molecular weight was determined by viscometery method. In-vitro hydrolysis study of valproic acid dextran conjugate was performed in different buffer solutions (pH 1.2, 7.4 and 9.0). The hydrolysis followed first order kinetics and a faster hydrolysis was observed at pH 9 compared to pH 7.4. The conjugate was evaluated for anticonvulsant, hepatotoxicity and ulcerogenic activities. The results showed a remarkable reduction in hepatotoxicity and ulcerogenicity along with comparable anti-convulsant activity as parent drug valproic acid.