Development and characterization of hematopoietic stem cells from human embryonic stem cells

Abstract

The hematopietic stem cells (HSCs) have the property of selfrenewal as well the ability to differentiate into all blood cell types (multilineage). The common sources of HSCs are bone marrow, peripheral blood and umbilical cord blood, but the quantity and compatibility of these HSCs is limited. HSCs obtained from human embryonic stem cells (hESCs) may provide better solution to the problems of quantity and compatibility. Generation of hematopoietic progenitors from hESCs need to be better understood and optimized in order to use them for fundamental research in the future. In the present study, differentiation of hESCs and colonies dissected from embryoid body (EB) into erythroid lineage was observed where the colonies were grown on human feeders and later transferred to feeder free media with high concentration of fibroblast growth factor (β-FGF). Moreover, in this study, hESCs from two blastocysts were spontaneously differentiated into hematopoietic cells. These colonies were characterized using immunophenotyping and karyotyping. Therefore, the fundamental knowledge of the biology of the cellular differentiation as described in this study will help us to better understand the reprogramming of induced pluripotent stem cells into hematopoietic stem cells for potential therapeutic use in the future. © 2021 Nova Science Publishers, Inc. All rights reserved.