Micropropagation and comparative phytochemical, antioxidant study of BacopaMonnieri (L.) Pennell

Abstract

Bacopa monnieri an important medicinal plant of Scorphulariaceae family and is distributed in the wet and marshy lands throughout India, Nepal, Sri Lanka, China, Taiwan, and Hawaii. It shows the presence of various phytoconstituents namely, alkaloids, saponins, phenolics and flavonoids. These phytoconstituents are responsible for anti oxidant and various other activity of the plant. The protocols for tissue culture has been developed for regeneration of the BM plant from small part (nodal segment). The regenerated plant having the similar potential in terms of phytoconstituents and antioxidant activity. The In vitro (leaf and stem) regenerated plants have slightly less antioxidant activity as compared to the In vivo but comparable. The protocols (MS+BAP5μg/ml) may used for the clonal regeneration of plant on large scale through which it can be protected and preserved for long duration in small space. A good response was observed in a soil ratio 3:2:1 (Soil rite: red sand: garden soil) for acclamatization.