Production of clinically efficient uricase enzyme induced from different strains of Pseudomonas aeruginosa under submerged fermentations and their kinetic properties

Abstract

Uricase is a therapeutic enzyme used to regulate the concentration of accumulated uric acid in gout disease. The two strains of Pseudomonas aeruginosa Ph3 & Pseudomonas aeruginosa 5Y2 had shown uricase production upon induction using uric acid (UA) as an inducer. At the optimized parameters, the crude preparations of strain 5Y2 showed comparatively higher uricase activity of 17.77 U/ml than strain Ph3 of 13.42 U/ml. The uricase production by 5Y2 was observed to be proportionally increased with the UA concentration; however, substrate inhibition was observed beyond the concentration of 8.92 mM of UA in the case of strain Ph3. The in vitro degradation study of urate crystals by Polarized Light Microscopy (PLM) showed the efficiency of both uricase. Kinetic parameters Km and Vmax for Ph3 uricase were found to be 9 µg/ml and 7.5 U/ml and for 5Y2 uricase those were 7 µg/ml and 7.77 U/ml. © 2016 Elsevier Ltd