Biodiesel production from hybrid non-edible oil using bio-support beads immobilized with lipase from Pseudomonas cepacia

Abstract

The main objective of this research was to check the feasibility of producing biodiesel from hybrid (Karanja and Castor) oil using bio-support beads immobilized with lipase from Pseudomonas cepacia. Karanja and Castor oil having an acid quantity of 14.71 mg KOH/g and 13.12 mg KOH/g, respectively, were added in equal amounts (50:50%). The fatty acid composition of the hybrid oil contained palmitic (13.4%), linoleic (2.9%), oleic (44.5%), stearic (2.2%) and ricinoleic acid (35.3%). Under the optimized conditions of enzymatic transesterification conditions, i.e. 2-propanol to oil molar ratio – 6:1, time – 24 h, enzyme (lipase) loading – 10% (w/w) and temperature – 50 ± 1 °C, the biodiesel yield was ∼78%. The reusability of immobilized lipase was tested for 12 cycles by taking 2-propanol as an acyl acceptor under standard conditions in the absence of solvent. After 9 cycles, the bio-support beads could retain up to ∼52% of its initial biodiesel yield. Furthermore, the properties the produced biodiesel were estimated using ASTM D6751 and confirmed by 1H NMR and FTIR analysis. © 2019 Elsevier Ltd