Antimicrobial evaluation of mangiferin and its synthesized analogues

Abstract

Objective: To isolate the mangiferin from Mangifera indica (M. indica) and assess the antimicrobial activity of different analogues synthesized from mangiferin. Methods: Mangiferin was isolated by column chromatography from the ethanolic extract of stem bark of M. indica. Mangiferin was further converted to 5-(N-phenylamino methyleno) mangiferin, 5-(N-p-chlorophenylamino methyleno) mangiferin, 5-(N-2-methyl phenylamino methyleno) mangiferin, 5-(N-p-methoxy phenylamino methyleno) mangiferin, 5-(N, N-diphenylamino methyleno) mangiferin, 5-(N-α-napthylamino methyleno) mangiferin and 5-(N-4-methyl phenylamino methyleno) mangiferin. Mangiferin and its analogues were characterized by melting point and Rf value determination and through spectral technique like UV, IR, and NMR spectral analysis. The antimicrobial effect of mangiferin and its derivatives was studied according to the disc diffusion method. Results: The solutions of mangiferin and its derivatives in polyethylene glycol-400 showed an activity with regard to four bacterial species- Bacillus pumilus (B. pumilus), Bacillus cereus (B. cereus) and Salmonella virchow (S. virchow) and two fungal species- Thermoascus aurantiacus (T. aurantiacus) and Aspergillus flavus (A. flavus). Conclusion: The present study confirms the antimicrobial activity of different analogues of mangiferin which could be further processed for the development of a potential antimicrobial agent. © 2012 Asian Pacific Tropical Biomedical Magazine.