Biosynthesized Silver Nanoparticles Having High Redox Current Enhance Anticancer Response for HepG2 Cells

Abstract

Silver nanoparticles (AgNPs) synthesized through the water extract of Trachyspermum ammi, Piper nigrum and Syzygium aromaticum had showed surface plasmon resonance in the range of 380 nm to 450 nm in UV–Vis spectra. Average size of these AgNPs was between 10 and 40 nm as observed in transmission electron microscopy image (TEM). High concentration of silver nitrate as a precursor showed an increase in the particle size, whereas the use of aqueous extract of Syzygium aromaticum exhibited high yield of AgNPs. Syzygium aromaticum synthesized AgNPs showed relatively increase in the oxidation peak current. The anticancer effect of these nanoparticles was investigated against HepG2 cells, and lowest IC50 value was observed in Syzygium aromaticum synthesized AgNPs as compared to the other two spices. Further, double staining by Hoechst 33,342/ PI dyes and DCFH-DA assay data unveiled that these nanoparticles induce apoptosis due to the generation of intracellular reactive oxygen species (ROS). The enhanced anticancer activities of Syzygium aromaticum synthesized AgNPs and corresponding increase in ROS. This may revealed that AgNPs with high redox current show the high anticancer properties. © 2022, The Author(s), under exclusive licence to Shiraz University.