Selection of metal salts for alkaline phosphatase production using response surface methodology

Abstract

Response surface methodology was used to optimize the concentration of important metal salts screened by Placket-Burman design. Eight metal salts such as CaCl2, CoCl2, CuSO4, FeCl3, MgSO4, MnSO4, NaCl and Pb(NO3)2 were tested by using Placket-Burman design criterion and four salts viz. CaCl2, MgSO4, MnSO4 and NaCl showed significant effect (P < 0.05) on alkaline phosphatase production. The metal salts concentration was optimized by applying central composite design (CCD) and the most suitable metal salts composition for alkaline phosphatase production in 100 ml medium was CaCl2 - 0.083 g; MgSO4 - 0.041 g; MnSO4 - 0.037 g and NaCl - 0.34 g. At these optimum levels of metal salts, alkaline phosphatase production of 2778.92 U/ml was obtained. 1.53-fold increase in the alkaline phosphatase yield was observed after selection and optimization of metal salts for alkaline phosphatase production by response surface methodology. © 2009 Elsevier Ltd. All rights reserved.