Synthesis, antibacterial and antifungal activities of N-mannich bases of 3-[N2-pyrimethaminylimino] isatin

Abstract

Schiff base of isatin with pyrimethamine and its N-Mannich bases were synthesized.Their chemical structures have been confirmed by UV, IR, 1H-NMR data and by elemental analyses. Antimicrobial evaluation was done by agar dilution method against 25 pathogenic bacteria and 6 pathogenic fungi. Among the new derivatives evaluated, 2-[1"-(morpholinomethyl)- 3"-isatinimino]-5-(4'-chlorophenyl)-6-ethyl-4-aminopyrimidine (PY4) exhibited higher potency compared to the standard drugs, against all bacteria. All the compounds exhibited antifungal activity.