Synthetic biology strategy for microbial cellulases: An overview

Abstract

Cellulase enzymes have a significant role in the biomass derived biofuels production process. Enzyme cost is one of the major challenges currently faced by biofuels industries. The biomass to biofuels production process is an environmentally friendly and sustainable approach. For developing economically viable cellulases, various approaches have been tried. Among these, synthetic biology is one of the potential techniques and is a sustainable approach to enhance the production of cellulase enzymes. Using recombinant expression technology, highly active and stable cellulases at low cost can be produced. Serious and in-depth investigations are under process to develop sustainable recombinant technology and other glycosyl-hydrolase enzymes.This chapter presents an overview of existing recombinant technology to enhance cellulase enzyme production. The latest recombinant methods in various cellulase producing microorganisms are discussed as well as these methods’ role in biofuels production processes from lignocellulosic biomass. Viable possibilities of recombinant technology using various methods are also explained in brief. © 2019 Elsevier B.V. All rights reserved.