Biomarker identification and gene-drug interaction prediction for breast cancer using machine learning algorithms

Abstract

Breast cancer (BC) poses a significant worldwide health challenge, necessitating the identification of its molecular origins and the development of possible treatment approaches. In this investigation, we introduce a pioneering methodology integrating genomic data and machine learning algorithms to identify genes associated with BC and their corresponding drugs. Initially, RNA-sequencing data of normal and malignant BC tissues publicly available in the NCBI GEO database were pre-processed using a standard pipeline. Further, machine learning algorithms, such as logistic regression, support vector machine, and random forest, were used to identify the differentially expressed genes (DEGs). The results were validated based on accuracy, sensitivity, specificity, precision, and F-score. Moreover, we identified the drugs corresponding to DEGs using the DepMap database. Our results revealed that genes such as OC90, KLK9, CXCL10, CDRT1, LCN6, GOLGA7B, and ZNF223 were commonly identified by all three machine learning algorithms. We found that the drugs DIHYDROROTENONE, 4-IODO-6-PHENYLPYRIMIDINE, BMS-754807, ARRY- 886, ESTRAMUSTINE-PHOSPHATE, FR-122047, and PIK 93 produced high correlation values on gene-drug interaction. The present study emphasizes the significance of utilizing genetic data and powerful machine learning algorithms to decipher the intricacies of BC biology and expedite the creation of tailored therapeutic approaches. © 2024 by Walter de Gruyter Berlin/Boston.