Effect of Gelucire based gastroretentive multiparticulates of metformin hydrochloride and glibenclamide on histology of diabetic rat pancreas

Abstract

The objective is to observe the effects of Gelucire based gastroretentive multiparticulates of metformin hydrochloride and glibenclamide on histology of diabetic rat pancreas. Diabetes was induced in wistar albino rats of either sex weighing 150-200g by streptozotocin. They were divided into different groups, Control group was given saline buffer only while other groups are orally administered with standard drugs (metformin hydrochloride and glibenclamide) separately, their combinations, Gelucire based gastroretentive multiparticulate formulations and their combination. Blood glucose level and physical health of rats were monitored regularly. After seven days of treatment, rats were sacrificed to isolate the pancreas for histopathological observation. The histopathological observation of pancreas shows that combination formulation of metformin hydrochloride and glibenclamide with Gelucire has remarkable effect on regeneration of pancreatic β- cells without any side effects. The formulations shown promising results and it is safe for diabetes type II treatment.