Evaluation of polyphenolic content and anti-oxidant potential of poly-herbal formulation

Abstract

In the present study a new poly-herbal formulation was prepared using fresh leaves of Lantana camara, Magnolia obovata, Albizzia lebbeck; stem bark of Clitoria ternatia,Cinnamomum cassia and dry fruits of Coriander sativum. Hydro-alcoholic (50:50) extract was prepared using soxhlet apparatus. Estimation of total phenol, tannin, flavonoid and flavonol content in the extract was performed. The extract contains phenol 2.37 mg/gm gallic acid equivalent (GAE) and tannin 0.96 mg/gm tannic acid equivalent (TAE). The flavanoid content was 8.20 mg/gm rutin equivalent (RE), and flavonol content was 6.98 mg/gm quercetrin equivalent (QE) in the extract. The total antioxidant activity of poly-herbal formulation extract was 0.217 absorption unit (au). Antioxidant estimation by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity was measured and result revealed that extract caused 50% inhibition at 104.74 µg/ml. Similarly, hydrogen peroxide scavenging activity was measured and EC50 of the extract was 46.89 µg/ml. Reducing power of the extract was found to be 0.454±0.014 µg/ml. The poly herbal formulation can be used as good source of natural anti-oxidant for health care and reducing the onset of oxidative induced health complications. © 2019, Research Trend. All rights reserved.