Fabrication and characterization of Nano Bioglass-ceramic scaffold for bone tissue engineering

Abstract

Fabrication of multifunctional scaffolds having good mechanical strength, tailored porosity, bioactivity and drug delivery ability is of great importance for bone tissue engineering. Bioactive glass (BG) with a system of SiO2-Na2O-CaO-P2O5-Ag2O-SrO was prepared by sol-gel method with different proportions of sucrose addition to develop porosity. The thermal behavior of the sample was analyzed using DTA and TGA. The sample was sintered at different temperatures to get optimum densification and the formed crystalline phases were identified using XRD. The morphology and structure of the prepared scaffolds were characterized by SEM and FTIR. Bioactivity of this glass was measured by the immersion of the sample in SBF for different time periods. The formation of hydroxy carbonate apatite layer was identified by FTIR, SEM and XRD which showed the presence of HCA as the main phase in the bioglass sample. The human blood compatibility of the sample was evaluated by hemolysis. The Young's modulus and compressive strength of bioglasses have been measured. Cell attachment, spreading and proliferation on the sample were determined using Human osteosarcoma U20S cells. The bioactivity, porosity, mechanical properties and biocompatibility of the glass samples make it a good potential prospect in the area of tissue engineering.